Biotechnologies of Crop Improvement, Volume 2 by Satbir Singh Gosal & Shabir Hussain Wani

Author:Satbir Singh Gosal & Shabir Hussain Wani
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

11.4.2.1 Copper Sulfate (CuSO4)

Of all the micronutrients, CuSO4 has gained utmost importance. It is known to play an important role in several metabolic activities like protein and carbohydrate metabolism (Kothari-Chajer et al. 2008); it is also directly involved in the photosynthetic electron transport chain as a constituent of plastocyanin, a copper-containing protein. Thus, copper might be affecting regeneration process by affecting photosynthesis process (Sahrawat and Chand 1999). The stimulatory effect of copper may be attributed to the fact that numerous copper-containing proteins naturally occur in plants, and it is a part of several enzymes and hence might play a key role on callus induction and regeneration. In conclusion, copper can be incorporated into MS basal medium to improve morphogenic response of explant.

In finger millet, many times an increase in regeneration response in the presence of elevated levels of copper was observed by Kothari et al. (2004), Kothari-Chajer et al. (2008), and Sharma et al. (2011). Kothari et al. (2004) studied the effect of micronutrient manipulation on callus induction and plant regeneration. CuSO4 increased to five times the normal concentration in the media resulted in a fourfold increase in number of regenerated shoots. Kothari-Chajer et al. (2008) found a marked improvement in quality, fresh weight, and percent response with increasing concentration of copper up to a threshold limit beyond which there is a decline in regeneration efficiency. Best regeneration response was observed at ten times Cu concentration; on the other hand, callus induced on medium devoid of Cu absolutely failed to regenerate. Similar results were reported by Sharma et al. (2011) where the presence of ten times CuSO4 in the media was found effective for enhancing the number of responding calli as well as for recovery of plantlets.

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